CoraLite® Plus 488-conjugated FER Monoclonal antibody
FER Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E3H2
Cat no : CL488-67671
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | NIH/3T3 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67671 targets FER in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | FER fusion protein Ag19176 相同性解析による交差性が予測される生物種 |
Full Name | fer (fps/fes related) tyrosine kinase |
Calculated molecular weight | 822 aa, 95 kDa |
Observed molecular weight | 95 kDa |
GenBank accession number | BC017060 |
Gene symbol | FER |
Gene ID (NCBI) | 2241 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 FER antibody CL488-67671 | Download protocol |
FC protocol for CL Plus 488 FER antibody CL488-67671 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |