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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGF-2 Polyclonal antibody, PBS Only

FGF-2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30333-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGF-2 Polyclonal antibody (30333-1-AP)

    WB analysis using 30333-1-AP (same clone as 30333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30333-1-AP (FGF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30333-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using FGF-2 Polyclonal antibody (30333-1-AP)

    IF Staining of SKOV-3 using 30333-1-AP (same clone as 30333-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using FGF-2 antibody (30333-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30333-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30333-1-PBS targets FGF-2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGF-2 fusion protein Ag28653 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor 2 (basic)
Calculated molecular weight 31 aa
Observed molecular weight21 kDa，23 kDa
GenBank accession numberNM_002006
Gene symbol FGF2
Gene ID (NCBI) 2247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.