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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGF19 Recombinant antibody

FGF19 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241740E4

Cat no : 84536-3-RR

Synonyms

UNQ334/PRO533, Fibroblast growth factor 19, FGF-19, FGF 19, 241740E4

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using FGF19 Recombinant antibody (84536-3-RR)

    WB analysis of HT-29 using 84536-3-RR

    HT-29 cells were subjected to SDS PAGE followed by western blot with 84536-3-RR (FGF19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84536-3-RR

    Affinity and Kinetic Characterization of 84536-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84536-3-RR against Human FGF19 were performed. The affinity constant is 0.323 nM.

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Tested Applications

Positive WB detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84536-3-RR targets FGF19 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FGF19 fusion protein HZ-1330 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor 19
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight24 kDa
GenBank accession numberBC017664
Gene symbol FGF19
Gene ID (NCBI) 9965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast growth factor 19 (FGF19) is a member of the fibroblast growth factor family, which consists of proteins with diverse effects on development, organogenesis, and metabolism. FGF19 is unique within this family as it functions as an endocrine protein, circulating in the blood and acting on distant target tissues. It is produced in the distal ileum and secreted into the circulation after a meal, playing a role in controlling the enterohepatic circulation of bile acids and regulating hepatic protein and glycogen metabolism in an insulin-independent manner. .

Protocols

Product Specific Protocols
WB protocol for FGF19 antibody 84536-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols