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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using FGF19 Recombinant antibody (84536-3-RR)

    WB analysis of HT-29 using 84536-3-RR (same clone as 84536-3-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 84536-3-RR (FGF19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84536-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01398-1

    Sandwich ELISA standard curve of MP01398-1

    Sandwich ELISA standard curve of MP01398-1, Human FGF19 Recombinant Matched Antibody Pair - PBS only. 84536-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1330. 84536-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-1000 pg/mL.

  • Sample test of MP01398-1

    Sample test of MP01398-1

    HT-29 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human FGF19, and measured 32.7 ng/mL.

  • Sample test of MP01398-1

    Sample test of MP01398-1

    Serum of six individual healthy human donors was measured. The FGF19 concentration of detected samples was determined to be 199.4 pg/mL with a range of 136.6-250.1 pg/mL.

  • Cytometric bead array standard curve of MP01398-1

    Cytometric bead array standard curve of MP01398-1

    Cytometric bead array standard curve of MP01398-1, FGF19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84536-3-PBS. Detection antibody: 84536-2-PBS. Standard: HZ-1330. Range: 0.078-20 ng/mL.

  • Affinity and Kinetic Characterization of 84536-3-RR

    Affinity and Kinetic Characterization of 84536-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84536-3-RR against Human FGF19 were performed. The affinity constant is 0.323 nM.

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FGF19 Recombinant antibody, PBS Only (Capture)

FGF19 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84536-3-PBS
Clone No.241740E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

UNQ334/PRO533, Fibroblast growth factor 19, FGF-19, FGF 19, 241740E4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84536-3-PBS targets FGF19 as part of a matched antibody pair:

MP01398-1: 84536-3-PBS capture and 84536-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FGF19 fusion protein HZ-1330 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor 19
Calculated molecular weight 216 aa, 24 kDa
Observed molecular weight24 kDa
GenBank accession numberBC017664
Gene Symbol FGF19
Gene ID (NCBI) 9965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95750
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fibroblast growth factor 19 (FGF19) is a member of the fibroblast growth factor family, which consists of proteins with diverse effects on development, organogenesis, and metabolism. FGF19 is unique within this family as it functions as an endocrine protein, circulating in the blood and acting on distant target tissues. It is produced in the distal ileum and secreted into the circulation after a meal, playing a role in controlling the enterohepatic circulation of bile acids and regulating hepatic protein and glycogen metabolism in an insulin-independent manner. .