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  • KD/KO Validated

FGFR3 Monoclonal antibody

FGFR3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3G1

Cat no : 66954-1-Ig

Synonyms

JTK4, HSFGFR3EX, FGFR 3, CEK2, CD333


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Tested Applications

Positive WB detected inLNCaP cells, L02 cells, L-929 cells, HeLa cells, HEK-293 cells, HepG2 cells, A549 cells, NCI-H1299 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66954-1-Ig targets FGFR3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FGFR3 fusion protein Ag26290 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor 3
Calculated molecular weight 87 kDa
Observed molecular weight 125-135 kDa
GenBank accession numberNM_000142
Gene symbol FGFR3
Gene ID (NCBI) 2261
RRIDAB_2882277
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3, and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, the FGFR3 gene has also been associated with tumor progression.

Protocols

Product Specific Protocols
WB protocol for FGFR3 antibody 66954-1-IgDownload protocol
IF protocol for FGFR3 antibody 66954-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Ann Rheum Dis

FGFR3 deficiency enhances CXCL12-dependent chemotaxis of macrophages via upregulating CXCR7 and aggravates joint destruction in mice.

Authors - Liang Kuang
  • KD Validated
humanWB

Cells

Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.

Authors - Damian Kołat
mouseWB

Bioengineered

The effects of Omarigliptin on promoting osteoblastic differentiation.

Authors - Fake Liao
humanWB

J Exp Clin Cancer Res

HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway.

Authors - Kai Guo
humanWB

J Exp Clin Cancer Res

Dysregulation and prometastatic function of glycosyltransferase C1GALT1 modulated by cHP1BP3/ miR-1-3p axis in bladder cancer.

Authors - Zengqi Tan
humanIF

Tissue Eng Part A

The UC-MSCbFGF/scaffold system accelerates the healing of the uterine full-thickness injury

Authors - Limin Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Clarisse (Verified Customer) (12-12-2022)

This antibody works very well on tissue fixed overnight at 4C with 2% PFA. No antigen retrieval required.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:750
  • Cell Tissue Type: Human cerebral organoids