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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGFR3 Monoclonal antibody (66954-1-Ig)

    WB analysis using 66954-1-Ig (same clone as 66954-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FGFR3 Monoclonal antibody (66954-1-Ig)

    WB analysis using 66954-1-Ig (same clone as 66954-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L-929 cells using FGFR3 Monoclonal antibody (66954-1-Ig)

    WB analysis of L-929 using 66954-1-Ig (same clone as 66954-1-PBS)

    L-929 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FGFR3 Monoclonal antibody (66954-1-Ig)

    WB analysis of HeLa using 66954-1-Ig (same clone as 66954-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FGFR3 Monoclonal antibody (66954-1-Ig)

    WB analysis of HEK-293 using 66954-1-Ig (same clone as 66954-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FGFR3 Monoclonal antibody (66954-1-Ig)

    WB analysis of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FGFR3 Monoclonal antibody (66954-1-Ig)

    IF Staining of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGFR3 antibody (66954-1-Ig, Clone: 1F3G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FGFR3 Monoclonal antibody (66954-1-Ig)

    IF Staining of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGFR3 antibody (66954-1-Ig, Clone: 1F3G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50578-1

    Cytometric bead array standard curve of MP50578-1

    Cytometric bead array standard curve of MP50578-1, FGFR3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66954-1-PBS. Detection antibody: 66954-2-PBS. Standard:Ag26290. Range: 1.563-200 ng/mL.

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  • Featured Product
  • KD/KO Validated

FGFR3 Monoclonal antibody, PBS Only (Capture)

FGFR3 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66954-1-PBS
Clone No.1F3G1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

JTK4, HSFGFR3EX, FGFR 3, CEK2, CD333

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66954-1-PBS targets FGFR3 as part of a matched antibody pair:

MP50578-1: 66954-1-PBS capture and 66954-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FGFR3 fusion protein Ag26290 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor 3
Calculated molecular weight 87 kDa
Observed molecular weight 125-135 kDa
GenBank accession numberNM_000142
Gene Symbol FGFR3
Gene ID (NCBI) 2261
RRIDAB_2882277
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3, and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, the FGFR3 gene has also been associated with tumor progression.