Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, Jurkat cells |
| Positive IF-P detected in | mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
67152-1-Ig targets FGL2 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FGL2 fusion protein Ag28619 相同性解析による交差性が予測される生物種 |
| Full Name | fibrinogen-like 2 |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC017813 |
| Gene Symbol | FGL2 |
| Gene ID (NCBI) | 10875 |
| RRID | AB_2882449 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14314 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation. The protein may play a role in physiologic functions at mucosal sites.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FGL2 antibody 67152-1-Ig | Download protocol |
| IF protocol for FGL2 antibody 67152-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Targeting fibrinogen-like protein 1 enhances immunotherapy in hepatocellular carcinoma | ||
Cell Rep FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation |