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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGL2 Monoclonal antibody, PBS Only

FGL2 Monoclonal Antibody for WB, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C9C5

Cat no : 67152-1-PBS

Synonyms

FGL2, FGL-2, fibrinogen like 2, Fibrinogen like protein 2, Fibroleukin, pT49, T49

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using FGL2 Monoclonal antibody (67152-1-Ig)

    WB analysis of THP-1 using 67152-1-Ig (same clone as 67152-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67152-1-Ig (FGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67152-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using FGL2 Monoclonal antibody (67152-1-Ig)

    WB analysis of Jurkat using 67152-1-Ig (same clone as 67152-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67152-1-Ig (FGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67152-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using FGL2 Monoclonal antibody (67152-1-Ig)

    IF Staining of mouse spleen using 67152-1-Ig (same clone as 67152-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using FGL2 antibody (67152-1-Ig, Clone: 2C9C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67152-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using FGL2 Monoclonal antibody (67152-1-Ig)

    IF Staining of mouse spleen using 67152-1-Ig (same clone as 67152-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using FGL2 antibody (67152-1-Ig, Clone: 2C9C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67152-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67152-1-PBS targets FGL2 in WB, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FGL2 fusion protein Ag28619 相同性解析による交差性が予測される生物種
Full Name fibrinogen-like 2
Calculated molecular weight 50 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC017813
Gene symbol FGL2
Gene ID (NCBI) 10875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation. The protein may play a role in physiologic functions at mucosal sites.