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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FIS1 Recombinant antibody (82248-1-RR)

    WB analysis using 82248-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82248-1-RR (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using FIS1 Recombinant antibody (82248-1-RR)

    IP experiment of HeLa using 82248-1-RR

    IP result of anti-FIS1 (IP:82248-1-RR, 4ug; Detection:82248-1-RR 1:5000) with HeLa cells lysate 1720 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FIS1 Recombinant antibody (82248-1-RR)

    IHC staining of human prostate cancer using 82248-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using FIS1 Recombinant antibody (82248-1-RR)

    IHC staining of rat brain using 82248-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FIS1 Recombinant antibody (82248-1-RR)

    IF Staining of HepG2 using 82248-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FIS1 antibody (82248-1-RR, Clone: 6N15 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using FIS1 Recombinant antibody (82248-1-RR)

    FC experiment of HepG2 using 82248-1-RR

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FIS1 (82248-1-RR, Clone:6N15) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82248-1-RR

    Affinity and Kinetic Characterization of 82248-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82248-1-RR against Human FIS1 were performed. The affinity constant is 0.251 nM.

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FIS1 Recombinant antibody

FIS1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 82248-1-RR
Clone No.6N15

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135, CGI 135

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82248-1-RR targets FIS1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FIS1 fusion protein Ag1409 相同性解析による交差性が予測される生物種
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated molecular weight 17 kDa
Observed molecular weight15 kDa
GenBank accession numberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
RRIDAB_3086472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3D6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (PMID: 21257639, 21459773, 23333625)

Protocols

Product Specific Protocols
WB protocol for FIS1 antibody 82248-1-RRDownload protocol
IHC protocol for FIS1 antibody 82248-1-RRDownload protocol
IF protocol for FIS1 antibody 82248-1-RRDownload protocol
IP protocol for FIS1 antibody 82248-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols