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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FIS1 Recombinant antibody

FIS1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

6N15

Cat no : 82248-1-RR

Synonyms

TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135, CGI 135

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FIS1 Recombinant antibody (82248-1-RR)

    WB analysis using 82248-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82248-1-RR (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using FIS1 Recombinant antibody (82248-1-RR)

    IP experiment of HeLa using 82248-1-RR

    IP result of anti-FIS1 (IP:82248-1-RR, 4ug; Detection:82248-1-RR 1:5000) with HeLa cells lysate 1720 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FIS1 Recombinant antibody (82248-1-RR)

    IHC staining of human prostate cancer using 82248-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using FIS1 Recombinant antibody (82248-1-RR)

    IHC staining of rat brain using 82248-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FIS1 Recombinant antibody (82248-1-RR)

    IF Staining of HepG2 using 82248-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FIS1 antibody (82248-1-RR, Clone: 6N15 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using FIS1 Recombinant antibody (82248-1-RR)

    FC experiment of HepG2 using 82248-1-RR

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FIS1 (82248-1-RR, Clone:6N15) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82248-1-RR

    Affinity and Kinetic Characterization of 82248-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82248-1-RR against Human FIS1 were performed. The affinity constant is 0.251 nM.

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Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82248-1-RR targets FIS1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FIS1 fusion protein Ag1409 相同性解析による交差性が予測される生物種
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated molecular weight 17 kDa
Observed molecular weight15 kDa
GenBank accession numberBC009428
Gene symbol FIS1
Gene ID (NCBI) 51024
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (PMID: 21257639, 21459773, 23333625)

Protocols

Product Specific Protocols
WB protocol for FIS1 antibody 82248-1-RRDownload protocol
IHC protocol for FIS1 antibody 82248-1-RRDownload protocol
IF protocol for FIS1 antibody 82248-1-RRDownload protocol
IP protocol for FIS1 antibody 82248-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols