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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FIS1 Recombinant antibody

FIS1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6N15

Cat no : CL488-82248

Synonyms

TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135, CGI 135

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated FIS1 Recombinant ant (CL488-82248)

    IF Staining of HepG2 using CL488-82248

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 FIS1 antibody (CL488-82248, Clone: 6N15 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated FIS1 Recombinant ant (CL488-82248)

    FC experiment of HepG2 using CL488-82248

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FIS1 (CL488-82248, Clone:6N15) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82248 targets FIS1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FIS1 fusion protein Ag1409 相同性解析による交差性が予測される生物種
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated molecular weight 17 kDa
Observed molecular weight15 kDa
GenBank accession numberBC009428
Gene symbol FIS1
Gene ID (NCBI) 51024
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (PMID: 21257639, 21459773, 23333625)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FIS1 antibody CL488-82248Download protocol
FC protocol for CL Plus 488 FIS1 antibody CL488-82248Download protocol
Standard Protocols
Click here to view our Standard Protocols