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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated FIS1 Monoclonal anti (CL488-66635)

    IF Staining of HepG2 using CL488-66635

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66635 (FIS1 antibody) at dilution of 1:50 .

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CoraLite® Plus 488-conjugated FIS1 Monoclonal antibody

FIS1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66635
Clone No.2F10E1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66635 targets FIS1 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FIS1 fusion protein Ag1409 相同性解析による交差性が予測される生物種
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated molecular weight 17 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
RRIDAB_2883363
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y3D6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FIS1 antibody CL488-66635Download protocol
Standard Protocols
Click here to view our Standard Protocols