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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus Mouse IgG2a Isotype Control (C1.18.4)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

C1.18.4

Cat no : FITC-65208

Synonyms

FITC Mouse IgG2a Isotype Control, IgG2a, IgG2a Isotype Control

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208)

    FC experiment of human PBMCs using FITC-65208

    1X10^6 human PBMCs were surface stained with 0.2 ug FITC Plus Anti-Mouse IgG2a Isotype Control (FITC-65208, Clone: C1.18.4) (red), or FITC Plus Anti-Human CD3 (OKT3) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of mouse splenocytes using FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208)

    FC experiment of mouse splenocytes using FITC-65208

    1X10^6 mouse splenocytes were surface stained with 0.2 ug FITC Anti-Mouse IgG2a Isotype Control (FITC-65208, Clone:C1.18.4) (red) or 0.2 ug FITC Anti-Mouse CD45.2 (FITC-65072) (green), or unstained (blue). Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs, mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65208 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name IgG2a Isotype Control
Gene symbol
Gene ID (NCBI)
RRIDAB_2883822
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The C1.18.4 immunoglobulin is useful as an isotype-matched control. The C.1.18.4 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for FITC Plus IgG2a Isotype Control antibody FITC-65208Download protocol
Standard Protocols
Click here to view our Standard Protocols