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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FKBP5 Monoclonal antibody (67874-1-Ig)

    WB analysis using 67874-1-Ig (same clone as 67874-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using FKBP5 Monoclonal antibody (67874-1-Ig)

    WB analysis of rat testis using 67874-1-Ig (same clone as 67874-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using FKBP5 Monoclonal antibody (67874-1-Ig)

    WB analysis of mouse testis using 67874-1-Ig (same clone as 67874-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FKBP5 Monoclonal antibody (67874-1-Ig)

    IHC staining of human colon cancer using 67874-1-Ig (same clone as 67874-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FKBP5 Monoclonal antibody (67874-1-Ig)

    IHC staining of human colon cancer using 67874-1-Ig (same clone as 67874-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

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FKBP5 Monoclonal antibody, PBS Only

FKBP5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67874-1-PBS
Clone No.3B11F3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67874-1-PBS targets FKBP5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FKBP5 fusion protein Ag5514 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 5
Calculated molecular weight 457 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC042605
Gene Symbol FKBP5
Gene ID (NCBI) 2289
RRIDAB_2918632
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13451
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).