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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FKBP5 Monoclonal antibody (67874-1-Ig)

    WB analysis using 67874-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of rat testis tissue using FKBP5 Monoclonal antibody (67874-1-Ig)

    WB analysis of rat testis using 67874-1-Ig

    rat testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using FKBP5 Monoclonal antibody (67874-1-Ig)

    WB analysis of mouse testis using 67874-1-Ig

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FKBP5 Monoclonal antibody (67874-1-Ig)

    IHC staining of human colon cancer using 67874-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FKBP5 Monoclonal antibody (67874-1-Ig)

    IHC staining of human colon cancer using 67874-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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FKBP5 Monoclonal antibody

FKBP5 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67874-1-Ig
Clone No.3B11F3
Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, rat testis tissue, mouse testis tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67874-1-Ig targets FKBP5 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FKBP5 fusion protein Ag5514 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 5
Calculated molecular weight 457 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC042605
Gene Symbol FKBP5
Gene ID (NCBI) 2289
RRIDAB_2918632
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13451
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).

Protocols

Product Specific Protocols
WB protocol for FKBP5 antibody 67874-1-IgDownload protocol
IHC protocol for FKBP5 antibody 67874-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Stem Cell Res Ther

Single-cell sequencing of facial adipose tissue unveils FKBP5 as a therapeutic target for facial infiltrating lipomatosis

Authors - Hongrui Chen
  • KD Validated
View Article