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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FKBP5 Monoclonal antibody

FKBP5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B11F3

Cat no : CL488-67874

Synonyms

Androgen-regulated protein 6, AIG6, 54 kDa progesterone receptor-associated immunophilin, 51 kDa FK506-binding protein, 3B11F3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated FKBP5 Monoclonal ant (CL488-67874)

    IF Staining of HeLa using CL488-67874

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 FKBP5 antibody (CL488-67874, Clone: 3B11F3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated FKBP5 Monoclonal ant (CL488-67874)

    FC experiment of K-562 using CL488-67874

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FKBP5 (CL488-67874, Clone:3B11F3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67874 targets FKBP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FKBP5 fusion protein Ag5514 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 5
Calculated molecular weight 457 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC042605
Gene symbol FKBP5
Gene ID (NCBI) 2289
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FKBP5 antibody CL488-67874Download protocol
Standard Protocols
Click here to view our Standard Protocols