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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FKBP52 Monoclonal antibody

FKBP52 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B5F1

Cat no : CL488-66040

Synonyms

FKBP4, FK506-binding protein 4, EC:5.2.1.8, 52 kDa FK506-binding protein, 51 kDa FK506-binding protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated FKBP52 Monoclonal an (CL488-66040)

    IF Staining of HeLa using CL488-66040

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 FKBP52 antibody (CL488-66040, Clone: 1B5F1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated FKBP52 Monoclonal an (CL488-66040)

    FC experiment of HeLa using CL488-66040

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FKBP52 (CL488-66040, Clone:1B5F1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66040 targets FKBP52 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FKBP52 fusion protein Ag18704 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 4, 59kDa
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC007924
Gene symbol FKBP52
Gene ID (NCBI) 2288
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FKBP52 antibody CL488-66040Download protocol
FC protocol for CL Plus 488 FKBP52 antibody CL488-66040Download protocol
Standard Protocols
Click here to view our Standard Protocols