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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of Jurkat using 66690-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis using 66690-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of HeLa using 66690-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of HEK-293 using 66690-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of HepG2 using 66690-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of NIH/3T3 using 66690-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of RAW 264.7 using 66690-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hypothalamus tissue using FKBP8 Monoclonal antibody (66690-1-Ig)

    IHC staining of human hypothalamus using 66690-1-Ig

    Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 66690-1-Ig (FKBP8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FKBP8 Monoclonal antibody (66690-1-Ig)

    IHC staining of mouse brain using 66690-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66690-1-Ig (FKBP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    IF Staining of A431 using 66690-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FKBP8 antibody (66690-1-Ig, Clone: 1C1G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  • Featured Product
  • KD/KO Validated

FKBP8 Monoclonal antibody

FKBP8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66690-1-Ig
Clone No.1C1G12
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman hypothalamus tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66690-1-Ig targets FKBP8 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FKBP8 fusion protein Ag1649 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 8, 38kDa
Calculated molecular weight 45 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC009966
Gene Symbol FKBP8
Gene ID (NCBI) 23770
RRIDAB_2882044
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for FKBP8 antibody 66690-1-IgDownload protocol
IHC protocol for FKBP8 antibody 66690-1-IgDownload protocol
IF protocol for FKBP8 antibody 66690-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

bioRxiv

Tau phosphorylation suppresses oxidative stress-induced mitophagy via FKBP8 receptor modulation

Authors - Michael O Isei
  • KD Validated
View Article
humanCoIP

J Biol Chem

Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma

Authors - Suli Lv
View Article