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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of Jurkat using 66690-1-Ig (same clone as 66690-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis using 66690-1-Ig (same clone as 66690-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of NIH/3T3 using 66690-1-Ig (same clone as 66690-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of RAW 264.7 using 66690-1-Ig (same clone as 66690-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of HeLa using 66690-1-Ig (same clone as 66690-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of HEK-293 using 66690-1-Ig (same clone as 66690-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    WB analysis of HepG2 using 66690-1-Ig (same clone as 66690-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hypothalamus tissue using FKBP8 Monoclonal antibody (66690-1-Ig)

    IHC staining of human hypothalamus using 66690-1-Ig (same clone as 66690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 66690-1-Ig (FKBP8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FKBP8 Monoclonal antibody (66690-1-Ig)

    IHC staining of mouse brain using 66690-1-Ig (same clone as 66690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66690-1-Ig (FKBP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FKBP8 Monoclonal antibody (66690-1-Ig)

    IF Staining of A431 using 66690-1-Ig (same clone as 66690-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FKBP8 antibody (66690-1-Ig, Clone: 1C1G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66690-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

FKBP8 Monoclonal antibody, PBS Only

FKBP8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66690-1-PBS
Clone No.1C1G12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66690-1-PBS targets FKBP8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FKBP8 fusion protein Ag1649 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 8, 38kDa
Calculated molecular weight 45 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC009966
Gene Symbol FKBP8
Gene ID (NCBI) 23770
RRIDAB_2882044
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14318
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.