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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis using 68491-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis of U2OS using 68491-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis of HEK-293 using 68491-1-Ig

    WB result of FLAD1 antibody (68491-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLAD1 transfected HEK-293 cells.

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FLAD1 Monoclonal antibody

FLAD1 Monoclonal Antibody for WB, ELISA
Cat No. 68491-1-Ig
Clone No.1F9A8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase, EC:2.7.7.2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, U2OS cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68491-1-Ig targets FLAD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FLAD1 fusion protein Ag6845 相同性解析による交差性が予測される生物種
Full Name FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
Calculated molecular weight 446 aa, 49 kDa
Observed molecular weight50 kDa
GenBank accession numberBC011378
Gene Symbol FLAD1
Gene ID (NCBI) 80308
RRIDAB_3085202
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NFF5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).

Protocols

Product Specific Protocols
WB protocol for FLAD1 antibody 68491-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols