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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis using 68491-1-Ig (same clone as 68491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis of U2OS using 68491-1-Ig (same clone as 68491-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FLAD1 Monoclonal antibody (68491-1-Ig)

    WB analysis of HEK-293 using 68491-1-Ig (same clone as 68491-1-PBS)

    WB result of FLAD1 antibody (68491-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLAD1 transfected HEK-293 cells. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

FLAD1 Monoclonal antibody, PBS Only

FLAD1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68491-1-PBS
Clone No.1F9A8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase, EC:2.7.7.2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68491-1-PBS targets FLAD1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FLAD1 fusion protein Ag6845 相同性解析による交差性が予測される生物種
Full Name FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
Calculated molecular weight 446 aa, 49 kDa
Observed molecular weight50 kDa
GenBank accession numberBC011378
Gene Symbol FLAD1
Gene ID (NCBI) 80308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NFF5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).