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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human FLT3/CD135 Rabbit Recombinant Antibody

FLT3/CD135 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241414F8

Cat no : 98106-1-RR

Synonyms

FLT3, FLK2, FL cytokine receptor, Fetal liver kinase-2, EC:2.7.10.1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Reh cells using Anti-Human FLT3/CD135 Rabbit Recombinant Antibody (98106-1-RR)

    FC experiment of Reh using 98106-1-RR

    1x10^6 Reh cells (left) or U-937 cells (right) were surface stained with 0.25 ug Anti-Human FLT3 Rabbit Recombinant Antibody (98106-1-RR, Clone:241414F8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98106-1-RR

    Affinity and Kinetic Characterization of 98106-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98106-1-RR against Human FLT3/CD135 were performed. The affinity constant is 1.25 nM.

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Tested Applications

Positive FC detected inReh cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98106-1-RR targets FLT3/CD135 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name fms-related tyrosine kinase 3
Calculated molecular weight 993 aa, 113 kDa
GenBank accession numberBC036028
Gene symbol FLT3
Gene ID (NCBI) 2322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Protocols

Product Specific Protocols
FC protocol for FLT3/CD135 antibody 98106-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols