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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555 Anti-Human FLT3/CD135 Rabbit Recombinant Antibody

FLT3/CD135 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

241414F8

Cat no : CL555-98106

Synonyms

FLT3, FLK2, FL cytokine receptor, Fetal liver kinase-2, EC:2.7.10.1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Reh cells using CoraLite® Plus 555 Anti-Human FLT3/CD135 Rabbit Re (CL555-98106)

    FC experiment of Reh using CL555-98106

    1x10^6 Reh cells (left) or U-937 cells (right) were surface stained with 5 ul CoraLite® Plus 555 Anti-Human FLT3/CD135 Rabbit Recombinant Antibody (CL555-98106, Clone: 241414F8) (red) or Isotype Control (blue). Cells were not fixed.

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Tested Applications

Positive FC detected inReh cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-98106 targets FLT3/CD135 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name fms-related tyrosine kinase 3
Calculated molecular weight 993 aa, 113 kDa
GenBank accession numberBC036028
Gene symbol FLT3
Gene ID (NCBI) 2322
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 FLT3/CD135 antibody CL555-98106Download protocol
Standard Protocols
Click here to view our Standard Protocols