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Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated FMR1 Monoclonal anti (CL488-66548)

    FC experiment of Jurkat using CL488-66548

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human FMR1 (CL488-66548, Clone:2B5B5) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO Validated

CoraLite® Plus 488-conjugated FMR1 Monoclonal antibody

FMR1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66548
Clone No.2B5B5

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

FC (Intra)

Synonyms

FMR1, FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1, FRAXA, POF, POF1, Protein FMR 1, 2B5B5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66548 targets FMR1 in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FMR1 fusion protein Ag4697 相同性解析による交差性が予測される生物種
Full Name fragile X mental retardation 1
Calculated molecular weight 632 aa, 71 kDa
Observed molecular weight 69-72 kDa
GenBank accession numberBC038998
Gene Symbol FMR1
Gene ID (NCBI) 2332
RRIDAB_3084257
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06787
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 FMR1 antibody CL488-66548Download protocol
Standard Protocols
Click here to view our Standard Protocols