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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FNTB Monoclonal antibody

FNTB Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F3B10

Cat no : CL488-66783

Synonyms

FTase-beta, FTase beta, FPTB, EC:2.5.1.58, 1F3B10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated FNTB Monoclonal anti (CL488-66783)

    IF Staining of A431 using CL488-66783

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 FNTB antibody (CL488-66783, Clone: 1F3B10 ) at dilution of 1:400.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated FNTB Monoclonal anti (CL488-66783)

    FC experiment of A431 using CL488-66783

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FNTB (CL488-66783, Clone:1F3B10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66783 targets FNTB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FNTB fusion protein Ag27796 相同性解析による交差性が予測される生物種
Full Name farnesyltransferase, CAAX box, beta
Calculated molecular weight 437 aa, 49 kDa
Observed molecular weight 44-45 kDa
GenBank accession numberBC020232
Gene symbol FNTB
Gene ID (NCBI) 2342
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FNTB antibody CL488-66783Download protocol
FC protocol for CL Plus 488 FNTB antibody CL488-66783Download protocol
Standard Protocols
Click here to view our Standard Protocols