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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXM1 Recombinant antibody (84681-1-RR)

    WB analysis using 84681-1-RR (same clone as 84681-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FOXM1 Recombinant antibody (84681-1-RR)

    WB analysis using 84681-1-RR (same clone as 84681-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01500-3

    Sandwich ELISA standard curve of MP01500-3

    Sandwich ELISA standard curve of MP01500-3, Human FOXM1 Recombinant Matched Antibody Pair - PBS only. 84681-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3729. 84681-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

  • Sample test of MP01500-3

    Sample test of MP01500-3

    The mean FOXM1 concentration was determined to be 3.3 ng/mL in NCI-H1299 cell extract based on a 1.5 mg/mL extract load.

  • Flow cytometry (FC) experiment of HeLa cells using FOXM1 Recombinant antibody (84681-1-RR)

    FC experiment of HeLa using 84681-1-RR (same clone as 84681-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using FOXM1 Recombinant antibody (84681-1-RR)

    FC experiment of A549 using 84681-1-RR (same clone as 84681-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01500-1

    Cytometric bead array standard curve of MP01500-1

    Cytometric bead array standard curve of MP01500-1, FOXM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84681-1-PBS. Detection antibody: 84681-2-PBS. Standard: Ag3729. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01500-2

    Cytometric bead array standard curve of MP01500-2

    Cytometric bead array standard curve of MP01500-2, FOXM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84681-1-PBS. Detection antibody: 84681-3-PBS. Standard: Ag3729. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84681-1-RR

    Affinity and Kinetic Characterization of 84681-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84681-1-RR against Human FOXM1 were performed. The affinity constant is 10.7 pM.

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FOXM1 Recombinant antibody, PBS Only (Capture/Detector)

FOXM1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84681-1-PBS
Clone No.242077A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16, FKHL16

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84681-1-PBS targets FOXM1 as part of a matched antibody pair:

MP01500-1: 84681-1-PBS capture and 84681-2-PBS detection (validated in Cytometric bead array)

MP01500-2: 84681-1-PBS capture and 84681-3-PBS detection (validated in Cytometric bead array)

MP01500-3: 84681-4-PBS capture and 84681-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FOXM1 fusion protein Ag3729 相同性解析による交差性が予測される生物種
Full Name forkhead box M 1
Calculated molecular weight 83 kDa
Observed molecular weight 84-110 kDa
GenBank accession numberBC006192
Gene Symbol FOXM1
Gene ID (NCBI) 2305
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08050
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly as5sociated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).