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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXM1 Recombinant antibody (84681-1-RR)

    WB analysis using 84681-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FOXM1 Recombinant antibody (84681-1-RR)

    WB analysis using 84681-1-RR

    L02 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using FOXM1 Recombinant antibody (84681-1-RR)

    FC experiment of HeLa using 84681-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of A549 cells using FOXM1 Recombinant antibody (84681-1-RR)

    FC experiment of A549 using 84681-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84681-1-RR

    Affinity and Kinetic Characterization of 84681-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84681-1-RR against Human FOXM1 were performed. The affinity constant is 10.7 pM.

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FOXM1 Recombinant antibody

FOXM1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84681-1-RR
Clone No.242077A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16, FKHL16

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, A431 cells, HT-29 cells, U2OS cells
Positive FC (Intra) detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84681-1-RR targets FOXM1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FOXM1 fusion protein Ag3729 相同性解析による交差性が予測される生物種
Full Name forkhead box M 1
Calculated molecular weight 83 kDa
Observed molecular weight 84-110 kDa
GenBank accession numberBC006192
Gene Symbol FOXM1
Gene ID (NCBI) 2305
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08050
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly as5sociated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).

Protocols

Product Specific Protocols
WB protocol for FOXM1 antibody 84681-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols