FOXN1 Monoclonal antibody
FOXN1 Monoclonal Antibody for WB, IHC, ELISA, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, ELISA, FC (Intra)
Conjugate
Unconjugated
CloneNo.
1D8B12
Cat no : 66337-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Raji cells, Jurkat cells |
Positive IHC detected in | mouse thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66337-1-Ig targets FOXN1 in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | FOXN1 fusion protein Ag18207 相同性解析による交差性が予測される生物種 |
Full Name | forkhead box N1 |
Calculated molecular weight | 648 aa, 69 kDa |
Observed molecular weight | 60 kDa |
GenBank accession number | BC146539 |
Gene symbol | FOXN1 |
Gene ID (NCBI) | 8456 |
RRID | AB_2881717 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality.
Protocols
Product Specific Protocols | |
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WB protocol for FOXN1 antibody 66337-1-Ig | Download protocol |
IHC protocol for FOXN1 antibody 66337-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |