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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FOXN1 Monoclonal antibody, PBS Only

FOXN1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D8B12

Cat no : 66337-1-PBS

Synonyms

Winged-helix transcription factor nude, WHN, RONU, Forkhead box protein N1, forkhead box N1

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using FOXN1 Monoclonal antibody (66337-1-Ig)

    WB analysis of Raji using 66337-1-Ig (same clone as 66337-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using FOXN1 Monoclonal antibody (66337-1-Ig)

    WB analysis of Jurkat using 66337-1-Ig (same clone as 66337-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using FOXN1 Monoclonal antibody (66337-1-Ig)

    IHC staining of mouse thymus using 66337-1-Ig (same clone as 66337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66337-1-Ig (FOXN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using FOXN1 Monoclonal antibody (66337-1-Ig)

    IHC staining of mouse thymus using 66337-1-Ig (same clone as 66337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66337-1-Ig (FOXN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using FOXN1 Monoclonal antibody (66337-1-Ig)

    FC experiment of A549 using 66337-1-Ig (same clone as 66337-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human FOXN1 (66337-1-Ig, Clone:1D8B12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 66337-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66337-1-PBS targets FOXN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FOXN1 fusion protein Ag18207 相同性解析による交差性が予測される生物種
Full Name forkhead box N1
Calculated molecular weight 648 aa, 69 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC146539
Gene symbol FOXN1
Gene ID (NCBI) 8456
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality.