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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using FRS2 Monoclonal antibody (66263-1-Ig)

    WB analysis of MCF-7 using 66263-1-Ig (same clone as 66263-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66263-1-Ig (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FRS2 Monoclonal antibody (66263-1-Ig)

    WB analysis of HEK-293 using 66263-1-Ig (same clone as 66263-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66263-1-Ig (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FRS2 Monoclonal antibody (66263-1-Ig)

    WB analysis of HeLa using 66263-1-Ig (same clone as 66263-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with (FRS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FRS2 Monoclonal antibody (66263-1-Ig)

    IHC staining of human prostate cancer using 66263-1-Ig (same clone as 66263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FRS2 Monoclonal antibody (66263-1-Ig)

    IHC staining of human prostate cancer using 66263-1-Ig (same clone as 66263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FRS2 Monoclonal antibody (66263-1-Ig)

    IF Staining of MCF-7 using 66263-1-Ig (same clone as 66263-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FRS2 antibody (66263-1-Ig, Clone: 4E10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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FRS2 Monoclonal antibody, PBS Only

FRS2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66263-1-PBS
Clone No.4E10H12

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SNT, FRS2alpha, FRS2A, FGFR-signaling adaptor SNT, FGFR substrate 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66263-1-PBS targets FRS2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen FRS2 fusion protein Ag19299 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor substrate 2
Calculated molecular weight 60 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC021562
Gene Symbol FRS2
Gene ID (NCBI) 10818
RRIDAB_2881650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WU20
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.