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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FRS2 Monoclonal antibody

FRS2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4E10H12

Cat no : 66263-1-Ig

Synonyms

FGFR signaling adaptor SNT, FGFR substrate 2, FRS2, FRS2A, FRS2alpha, SNT, SNT 1, SNT1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using FRS2 Monoclonal antibody (66263-1-Ig)

    WB analysis of MCF-7 using 66263-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66263-1-Ig (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FRS2 Monoclonal antibody (66263-1-Ig)

    WB analysis of HEK-293 using 66263-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66263-1-Ig (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FRS2 Monoclonal antibody (66263-1-Ig)

    WB analysis of HeLa using 66263-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with (FRS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FRS2 Monoclonal antibody (66263-1-Ig)

    IHC staining of human prostate cancer using 66263-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FRS2 Monoclonal antibody (66263-1-Ig)

    IHC staining of human prostate cancer using 66263-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FRS2 Monoclonal antibody (66263-1-Ig)

    IF Staining of MCF-7 using 66263-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using FRS2 antibody (66263-1-Ig, Clone: 4E10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66263-1-Ig targets FRS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen FRS2 fusion protein Ag19299 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor substrate 2
Calculated molecular weight 60 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC021562
Gene symbol FRS2
Gene ID (NCBI) 10818
RRIDAB_2881650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.

Protocols

Product Specific Protocols
WB protocol for FRS2 antibody 66263-1-IgDownload protocol
IHC protocol for FRS2 antibody 66263-1-IgDownload protocol
IF protocol for FRS2 antibody 66263-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols