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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FRS2 Monoclonal antibody

FRS2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4E10H12

Cat no : CL488-66263

Synonyms

SNT, FRS2alpha, FRS2A, FGFR-signaling adaptor SNT, FGFR substrate 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated FRS2 Monoclonal anti (CL488-66263)

    IF Staining of MCF-7 using CL488-66263

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CL488-66263 (FRS2 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated FRS2 Monoclonal anti (CL488-66263)

    FC experiment of MCF-7 using CL488-66263

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FRS2 (CL488-66263, Clone:4E10H12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66263 targets FRS2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen FRS2 fusion protein Ag19299 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor substrate 2
Calculated molecular weight 60 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC021562
Gene symbol FRS2
Gene ID (NCBI) 10818
RRIDAB_2883296
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FRS2 antibody CL488-66263Download protocol
Standard Protocols
Click here to view our Standard Protocols