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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FRZB Monoclonal antibody

FRZB Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B4A1

Cat no : CL488-67081

Synonyms

Frizzled related protein 1, FRITZ, Frezzled, FRE, FIZ

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Neuro-2a cells using CoraLite® Plus 488-conjugated FRZB Monoclonal anti (CL488-67081)

    FC experiment of Neuro-2a using CL488-67081

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FRZB (CL488-67081, Clone:2B4A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67081 targets FRZB in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FRZB fusion protein Ag28147 相同性解析による交差性が予測される生物種
Full Name frizzled-related protein
Calculated molecular weight 325 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC027855
Gene symbol FRZB
Gene ID (NCBI) 2487
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Frzb (also known as sFRP3) is a member of the secreted frizzled related protein (SFRP) family, originally identified as a chondrogenic factor during bone morphogenesis (PMID: 29209231). It was subsequently shown to be a regulator in Wnt signaling, and also have a role in regulating cell growth and differentiation in specific cell types. FRZB was seen to be acting as an oncogene in some cancer, such as metastatic renal cancer.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 FRZB antibody CL488-67081Download protocol
Standard Protocols
Click here to view our Standard Protocols