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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Ferritin heavy chain Recombinant antibody (83428-1-RR)

    WB analysis of HeLa using 83428-1-RR (same clone as 83428-1-PBS)

    WB result of Ferritin heavy chain antibody (83428-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells. This data was developed using the same antibody clone with 83428-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Ferritin heavy chain Recombinant antibody (83428-1-RR)

    WB analysis using 83428-1-RR (same clone as 83428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83428-1-RR (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83428-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ferritin heavy chain Recombinant antibody (83428-1-RR)

    IF Staining of HeLa using 83428-1-RR (same clone as 83428-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (83428-1-RR, Clone: 230312F3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83428-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83428-1-RR

    Affinity and Kinetic Characterization of 83428-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83428-1-RR against Human FTH1 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

Ferritin heavy chain Recombinant antibody, PBS Only

Ferritin heavy chain Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83428-1-PBS
Clone No.230312F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Ferritin, FTH1, FTH, Ferritin heavy chain, N-terminally processed, EC:1.16.3.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83428-1-PBS targets Ferritin heavy chain in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ferritin heavy chain fusion protein Ag2290 相同性解析による交差性が予測される生物種
Full Name ferritin, heavy polypeptide 1
Calculated molecular weight 183 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC000857
Gene Symbol FTH1
Gene ID (NCBI) 2495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02794
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.