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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ferritin heavy chain Recombinant antibody

Ferritin heavy chain Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230312F3

Cat no : 83428-1-RR

Synonyms

Ferritin H subunit, Ferritin heavy chain, ferritin, heavy polypeptide 1, FHC, FTH, FTH1, FTHL6, PIG15, PLIF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ferritin heavy chain Recombinant antibody (83428-1-RR)

    WB analysis using 83428-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83428-1-RR (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83428-1-RR

    Affinity experiments of 83428-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83428-1-RR against Human FTH1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, human liver tissue, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83428-1-RR is Ferritin heavy chain Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ferritin heavy chain fusion protein Ag2290 相同性解析による交差性が予測される生物種
Full Name ferritin, heavy polypeptide 1
Calculated molecular weight 183 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC000857
Gene symbol FTH1
Gene ID (NCBI) 2495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

Protocols

Product Specific Protocols
WB protocol for Ferritin heavy chain antibody 83428-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols