FTO Monoclonal antibody
FTO Monoclonal Antibody for WB, IHC, ELISA, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Pig and More (3)
Applications
WB, IHC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1E8B1
Cat no : 68111-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | LNCaP cells, NIH/3T3 cells, pig brain tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | mouse brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
68111-1-Ig targets FTO in WB, IHC, FC (Intra), ELISA applications and shows reactivity with Human, Mouse, Pig samples.
| Tested Reactivity | Human, Mouse, Pig |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FTO fusion protein Ag26095 相同性解析による交差性が予測される生物種 |
| Full Name | fat mass and obesity associated |
| Calculated molecular weight | 58 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | NM_001080432 |
| Gene symbol | FTO |
| Gene ID (NCBI) | 79068 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FTO antibody 68111-1-Ig | Download protocol |
| IHC protocol for FTO antibody 68111-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol METTL3 enhances E. coli F18 resistance by targeting IKBKG/NF-κB signaling via an m6A-YTHDF1-dependent manner in IPEC-J2 cells | ||
Acta Physiol (Oxf) Landscape of transcriptome-wide m6A modification in diabetic liver reveals rewiring of PI3K-Akt signaling after physical exercise | ||
Apoptosis NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer |
