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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FTO Monoclonal antibody (68111-1-Ig)

    WB analysis using 68111-1-Ig (same clone as 68111-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using FTO Monoclonal antibody (68111-1-Ig)

    WB analysis of NIH/3T3 using 68111-1-Ig (same clone as 68111-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FTO Monoclonal antibody (68111-1-Ig)

    IHC staining of mouse brain using 68111-1-Ig (same clone as 68111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FTO Monoclonal antibody (68111-1-Ig)

    IHC staining of human liver cancer using 68111-1-Ig (same clone as 68111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FTO Monoclonal antibody (68111-1-Ig)

    IHC staining of human liver cancer using 68111-1-Ig (same clone as 68111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FTO Monoclonal antibody (68111-1-Ig)

    IHC staining of mouse brain using 68111-1-Ig (same clone as 68111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using FTO Monoclonal antibody (68111-1-Ig)

    FC experiment of SH-SY5Y using 68111-1-Ig (same clone as 68111-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (68111-1-Ig, Clone:1E8B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

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FTO Monoclonal antibody, PBS Only

FTO Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 68111-1-PBS
Clone No.1E8B1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

1E8B1, KIAA1752

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68111-1-PBS targets FTO in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FTO fusion protein Ag26095 相同性解析による交差性が予測される生物種
Full Name fat mass and obesity associated
Calculated molecular weight 58 kDa
Observed molecular weight58 kDa
GenBank accession numberNM_001080432
Gene Symbol FTO
Gene ID (NCBI) 79068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9C0B1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.