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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FTO Recombinant antibody, PBS Only

FTO Recombinant Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4L4

Cat no : 81471-1-PBS

Synonyms

FTO, KIAA1752

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FTO Recombinant antibody (81471-1-RR)

    WB analysis using 81471-1-RR (same clone as 81471-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81471-1-RR (FTO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FTO Recombinant antibody (81471-1-RR)

    IHC staining of human liver cancer using 81471-1-RR (same clone as 81471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81471-1-RR (FTO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FTO Recombinant antibody (81471-1-RR)

    IHC staining of human liver cancer using 81471-1-RR (same clone as 81471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81471-1-RR (FTO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FTO Recombinant antibody (81471-1-RR)

    IF Staining of HepG2 using 81471-1-RR (same clone as 81471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FTO antibody (81471-1-RR, Clone: 4L4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81471-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81471-1-PBS targets FTO in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FTO fusion protein Ag26095 相同性解析による交差性が予測される生物種
Full Name fat mass and obesity associated
Calculated molecular weight 58 kDa
Observed molecular weight58 kDa
GenBank accession numberNM_001080432
Gene symbol FTO
Gene ID (NCBI) 79068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.