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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FTO Recombinant antibody

FTO Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4L4

Cat no : 81471-1-RR

Synonyms

FTO, KIAA1752

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FTO Recombinant antibody (81471-1-RR)

    WB analysis using 81471-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81471-1-RR (FTO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FTO Recombinant antibody (81471-1-RR)

    IHC staining of human liver cancer using 81471-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81471-1-RR (FTO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FTO Recombinant antibody (81471-1-RR)

    IHC staining of human liver cancer using 81471-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81471-1-RR (FTO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FTO Recombinant antibody (81471-1-RR)

    IF Staining of HepG2 using 81471-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FTO antibody (81471-1-RR, Clone: 4L4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, LNCaP cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81471-1-RR targets FTO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FTO fusion protein Ag26095 相同性解析による交差性が予測される生物種
Full Name fat mass and obesity associated
Calculated molecular weight 58 kDa
Observed molecular weight58 kDa
GenBank accession numberNM_001080432
Gene symbol FTO
Gene ID (NCBI) 79068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

Protocols

Product Specific Protocols
WB protocol for FTO antibody 81471-1-RRDownload protocol
IHC protocol for FTO antibody 81471-1-RRDownload protocol
IF protocol for FTO antibody 81471-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols