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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FUCA2 Polyclonal antibody, PBS Only

FUCA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30080-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FUCA2 Polyclonal antibody (30080-1-AP)

    WB analysis using 30080-1-AP (same clone as 30080-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 30080-1-AP (FUCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FUCA2 Polyclonal antibody (30080-1-AP)

    IHC staining of human stomach cancer using 30080-1-AP (same clone as 30080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30080-1-AP (FUCA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FUCA2 Polyclonal antibody (30080-1-AP)

    IHC staining of human stomach cancer using 30080-1-AP (same clone as 30080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30080-1-AP (FUCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FUCA2 Polyclonal antibody (30080-1-AP)

    IF Staining of HepG2 using 30080-1-AP (same clone as 30080-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FUCA2 antibody (30080-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30080-1-PBS targets FUCA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUCA2 fusion protein Ag32467 相同性解析による交差性が予測される生物種
Full Name fucosidase, alpha-L- 2, plasma
Calculated molecular weight 54 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC003060
Gene symbol FUCA2
Gene ID (NCBI) 2519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.