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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FUS/TLS Monoclonal antibody

FUS/TLS Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B4F8

Cat no : CL488-68262

Synonyms

FUS, ALS6, ALS, 75 kDa DNA-pairing protein, 75 kDa DNA pairing protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated FUS/TLS Monoclonal a (CL488-68262)

    IF Staining of mouse brain using CL488-68262

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 FUS/TLS antibody (CL488-68262, Clone: 1B4F8 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated FUS/TLS Monoclonal a (CL488-68262)

    IF Staining of mouse brain using CL488-68262

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 FUS/TLS antibody (CL488-68262, Clone: 1B4F8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated FUS/TLS Monoclonal a (CL488-68262)

    FC experiment of K-562 using CL488-68262

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FUS/TLS (CL488-68262, Clone:1B4F8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68262 targets FUS/TLS in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FUS/TLS fusion protein Ag2150 相同性解析による交差性が予測される生物種
Full Name fusion (involved in t(12;16) in malignant liposarcoma)
Calculated molecular weight 75 kDa
Observed molecular weight 68-75 kDa
GenBank accession numberBC026062
Gene symbol FUS/TLS
Gene ID (NCBI) 2521
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)(PMID: 22640227). Multiple phosphorylation on the N terminus of FUS caused that FUS was detected 68-75 kDa (PMID:24899704).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FUS/TLS antibody CL488-68262Download protocol
FC protocol for CL Plus 488 FUS/TLS antibody CL488-68262Download protocol
Standard Protocols
Click here to view our Standard Protocols