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- KD/KO Validated
FUT8 Monoclonal antibody
FUT8 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2F7H2
Cat no : 66118-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MDA-MB-231 cells, MDA-MB-453s cells, MCF-7 cells, Caco-2 cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 5 publications below |
| IF | See 2 publications below |
Product Information
66118-1-Ig targets FUT8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FUT8 fusion protein Ag18769 相同性解析による交差性が予測される生物種 |
| Full Name | fucosyltransferase 8 (alpha (1,6) fucosyltransferase) |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC025385 |
| Gene symbol | FUT8 |
| Gene ID (NCBI) | 2530 |
| RRID | AB_2881517 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FUT8 antibody 66118-1-Ig | Download protocol |
| IHC protocol for FUT8 antibody 66118-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell A Systems Biology Approach Identifies FUT8 as a Driver of Melanoma Metastasis.
| ||
Nat Commun EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses | ||
Am J Hum Genet Biallelic Mutations in FUT8 Cause a Congenital Disorder of Glycosylation with Defective Fucosylation. | ||
Mol Cell Proteomics Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei. | ||
Am J Cancer Res Fucosyltransferase 8 modulates receptor tyrosine kinase activation and temozolomide resistance in glioblastoma cells.
| ||
Mol Cell Proteomics Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei |
