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Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-231 cells using FUT8 Monoclonal antibody (66118-1-Ig)

    WB analysis of MDA-MB-231 using 66118-1-Ig (same clone as 66118-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using FUT8 Monoclonal antibody (66118-1-Ig)

    WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

    WB result of FUT8 antibody (66118-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUT8 transfected MCF-7 cells. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-453s cells using FUT8 Monoclonal antibody (66118-1-Ig)

    WB analysis of MDA-MB-453s using 66118-1-Ig (same clone as 66118-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using FUT8 Monoclonal antibody (66118-1-Ig)

    WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using FUT8 Monoclonal antibody (66118-1-Ig)

    WB analysis of Caco-2 using 66118-1-Ig (same clone as 66118-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FUT8 Monoclonal antibody (66118-1-Ig)

    IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FUT8 Monoclonal antibody (66118-1-Ig)

    IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FUT8 Monoclonal antibody (66118-1-Ig)

    IHC staining of human lung cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 10x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FUT8 Monoclonal antibody (66118-1-Ig)

    IHC staining of human lung cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 40x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FUT8 Monoclonal antibody (66118-1-Ig)

    IHC staining of human breast cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 40x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FUT8 Monoclonal antibody (66118-1-Ig)

    IHC staining of human breast cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66118-1-Ig (FUT8 Antibody) at dilution of 1:50 under 10x lens. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

FUT8 Monoclonal antibody, PBS Only

FUT8 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66118-1-PBS
Clone No.2F7H2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Alpha (1,6) fucosyltransferase, alpha1 6FucT, Fucosyltransferase 8, FUT8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66118-1-PBS targets FUT8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FUT8 fusion protein Ag18769 相同性解析による交差性が予測される生物種
Full Name fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC025385
Gene Symbol FUT8
Gene ID (NCBI) 2530
RRIDAB_2881517
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BYC5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).