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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FXR1 Monoclonal antibody (67813-1-Ig)

    WB analysis using 67813-1-Ig (same clone as 67813-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67813-1-Ig (FXR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using FXR1 Monoclonal antibody (67813-1-Ig)

    IP experiment of K-562 using 67813-1-Ig (same clone as 67813-1-PBS)

    IP result of anti-FXR1 (IP:67813-1-Ig, 5ug; Detection:67813-1-Ig 1:1000) with K-562 cells lysate 3680 ug. This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FXR1 Monoclonal antibody (67813-1-Ig)

    IHC staining of mouse brain using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using FXR1 Monoclonal antibody (67813-1-Ig)

    IHC staining of human gliomas using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using FXR1 Monoclonal antibody (67813-1-Ig)

    IHC staining of human gliomas using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FXR1 Monoclonal antibody (67813-1-Ig)

    IHC staining of mouse brain using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using FXR1 Monoclonal antibody (67813-1-Ig)

    IF Staining of mouse brain using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FXR1 Monoclonal antibody (67813-1-Ig)

    IF Staining of HeLa using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FXR1 Monoclonal antibody (67813-1-Ig)

    IF Staining of HeLa using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

FXR1 Monoclonal antibody, PBS Only

FXR1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 67813-1-PBS
Clone No.1E12E8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1, 1E12E8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67813-1-PBS targets FXR1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FXR1 fusion protein Ag27800 相同性解析による交差性が予測される生物種
Full Name fragile X mental retardation, autosomal homolog 1
Calculated molecular weight 621 aa, 70 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC028983
Gene Symbol FXR1
Gene ID (NCBI) 8087
RRIDAB_2918576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51114
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.