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FXR1 Monoclonal antibody
FXR1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1E12E8
Cat no : 67813-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
67813-1-Ig targets FXR1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FXR1 fusion protein Ag27800 相同性解析による交差性が予測される生物種 |
| Full Name | fragile X mental retardation, autosomal homolog 1 |
| Calculated molecular weight | 621 aa, 70 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC028983 |
| Gene symbol | FXR1 |
| Gene ID (NCBI) | 8087 |
| RRID | AB_2918576 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FXR1 antibody 67813-1-Ig | Download protocol |
| IHC protocol for FXR1 antibody 67813-1-Ig | Download protocol |
| IF protocol for FXR1 antibody 67813-1-Ig | Download protocol |
| IP protocol for FXR1 antibody 67813-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Pharmacol Silibinin, a commonly used therapeutic agent for non-alcohol fatty liver disease, functions through upregulating intestinal expression of fibroblast growth factor 15/19 | ||
Oncol Lett FXR1 promotes proliferation, invasion and migration of hepatocellular carcinoma in vitro and in vivo
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