- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated FXR1 Monoclonal antibody
FXR1 Monoclonal Antibody for IF/ICC, IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC, IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E12E8
Cat no : CL488-67813
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67813 targets FXR1 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FXR1 fusion protein Ag27800 相同性解析による交差性が予測される生物種 |
| Full Name | fragile X mental retardation, autosomal homolog 1 |
| Calculated molecular weight | 621 aa, 70 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC028983 |
| Gene symbol | FXR1 |
| Gene ID (NCBI) | 8087 |
| RRID | AB_2919554 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MV 69 kDa and 60 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FXR1 antibody CL488-67813 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
