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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FXYD1 Monoclonal antibody

FXYD1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2C10G9

Cat no : 67150-1-Ig

Synonyms

FXYD1, Phospholemman, PLM

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    WB analysis of human heart using 67150-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    WB analysis of human skeletal muscle using 67150-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    WB analysis of pig heart using 67150-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    IHC staining of human heart using 67150-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    IHC staining of human heart using 67150-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    IF Staining of mouse heart using 67150-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    IF Staining of mouse heart using 67150-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman heart tissue, human skeletal muscle tissue, pig heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67150-1-Ig targets FXYD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, mouse samples.

Tested Reactivity Human, Pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FXYD1 fusion protein Ag25307 相同性解析による交差性が予測される生物種
Full Name FXYD domain containing ion transport regulator 1
Calculated molecular weight 92 aa, 10 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC032800
Gene symbol FXYD1
Gene ID (NCBI) 5348
RRIDAB_2882448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

Protocols

Product Specific Protocols
WB protocol for FXYD1 antibody 67150-1-IgDownload protocol
IHC protocol for FXYD1 antibody 67150-1-IgDownload protocol
IF protocol for FXYD1 antibody 67150-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols