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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    WB analysis of human heart using 67150-1-Ig (same clone as 67150-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    WB analysis of human skeletal muscle using 67150-1-Ig (same clone as 67150-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    WB analysis of pig heart using 67150-1-Ig (same clone as 67150-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    IHC staining of human heart using 67150-1-Ig (same clone as 67150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    IHC staining of human heart using 67150-1-Ig (same clone as 67150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    IF Staining of mouse heart using 67150-1-Ig (same clone as 67150-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using FXYD1 Monoclonal antibody (67150-1-Ig)

    IF Staining of mouse heart using 67150-1-Ig (same clone as 67150-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

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FXYD1 Monoclonal antibody, PBS Only

FXYD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67150-1-PBS
Clone No.2C10G9

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

FXYD1, Phospholemman, PLM

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67150-1-PBS targets FXYD1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, mouse samples.

Tested Reactivity Human, Pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FXYD1 fusion protein Ag25307 相同性解析による交差性が予測される生物種
Full Name FXYD domain containing ion transport regulator 1
Calculated molecular weight 92 aa, 10 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC032800
Gene Symbol FXYD1
Gene ID (NCBI) 5348
RRIDAB_2882448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00168
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)