CoraLite® Plus 488-conjugated Factor X Monoclonal antibody
Factor X Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3D3F11
Cat no : CL488-66753
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66753 targets Factor X in IF-P applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Factor X fusion protein Ag11664 相同性解析による交差性が予測される生物種 |
| Full Name | coagulation factor X |
| Calculated molecular weight | 488 aa, 55 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC046125 |
| Gene symbol | F10 |
| Gene ID (NCBI) | 2159 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Coagulation factor X (F10) is a vitamin K-dependent glycoprotein that participates in the middle phase of the blood coagulation cascade (PMID: 1931959; 1313796). It is synthesized as a single-chain precursor in the liver where it undergoes a number of covalent processing steps before secretion into the blood as a two-chain molecule linked by a disulfide bond (PMID: 1313796). The light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. Factor X is activated into factor Xa, by both the extrinsic and intrinsic pathway. The activated factor then converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Factor X antibody CL488-66753 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
