CoraLite® Plus 488-conjugated Fas/CD95 Recombinant antibody
Fas/CD95 Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240340E11
Cat no : CL488-83400-5
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL488-83400-5 is Fas/CD95 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Fusion Protein 相同性解析による交差性が予測される生物種 |
Full Name | Fas (TNF receptor superfamily, member 6) |
Calculated molecular weight | 38 kDa |
GenBank accession number | NM_000043.6 |
Gene symbol | CD95 |
Gene ID (NCBI) | 355 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Fas/CD95 antibody CL488-83400-5 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |