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  • Featured Product
  • KD/KO Validated

Fascin Monoclonal antibody, PBS Only

Fascin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5G3

Cat no : 66321-1-PBS

Synonyms

FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using Fascin Monoclonal antibody (66321-1-Ig)

    WB analysis of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using Fascin Monoclonal antibody (66321-1-Ig)

    WB analysis of K-562 using 66321-1-Ig (same clone as 66321-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Fascin Monoclonal antibody (66321-1-Ig)

    WB analysis of HeLa using 66321-1-Ig (same clone as 66321-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Fascin Monoclonal antibody (66321-1-Ig)

    IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Fascin Monoclonal antibody (66321-1-Ig)

    IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Fascin Monoclonal antibody (66321-1-Ig)

    IHC staining of human pancreas cancer using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Fascin Monoclonal antibody (66321-1-Ig)

    IHC staining of human skin cancer using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Fascin Monoclonal antibody (66321-1-Ig)

    IHC staining of human skin cancer using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Fascin Monoclonal antibody (66321-1-Ig)

    IHC staining of human pancreas cancer using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Fascin Monoclonal antibody (66321-1-Ig)

    IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66321-1-Ig (Fascin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Fascin Monoclonal antibody (66321-1-Ig)

    IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Fascin antibody (66321-1-Ig, Clone: 1B5G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Fascin Monoclonal antibody (66321-1-Ig)

    IF Staining of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66321-1-Ig(Fascin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Fascin Monoclonal antibody (66321-1-Ig)

    FC experiment of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Fascin Monoclonal antibody (66321-1-Ig, Clone:1B5G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66321-1-PBS targets Fascin in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Fascin fusion protein Ag6074 相同性解析による交差性が予測される生物種
Full Name fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC000521
Gene symbol Fascin
Gene ID (NCBI) 6624
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.